Lipidic cubic phase technologies for membrane protein structural studies
نویسندگان
چکیده
منابع مشابه
Femtosecond crystallography of membrane proteins in the lipidic cubic phase.
Despite recent technological advances in heterologous expression, stabilization and crystallization of membrane proteins (MPs), their structural studies remain difficult and require new transformative approaches. During the past two years, crystallization in lipidic cubic phase (LCP) has started gaining a widespread acceptance, owing to the spectacular success in high-resolution structure deter...
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School of Chemistry, Bio21 Institute, Unive CSIRO Manufacturing Flagship, Bag 10, Cla [email protected] School of Science, College of Science, En Melbourne, VIC 3000, Australia † Electronic supplementary informatio parameters for top vs. bottom well (Fig from tting a rst order decay function t gA0 concentration (mol%) (Table S1); c encapsulated in monoolein and phytantr combination of the ...
متن کاملA Plug-Based Microfluidic System for Dispensing Lipidic Cubic Phase (LCP) Material Validated by Crystallizing Membrane Proteins in Lipidic Mesophases.
This paper presents a plug-based microfluidic system to dispense nanoliter-volume plugs of Lipidic Cubic Phase (LCP) material and subsequently merge the LCP plugs with aqueous plugs. This system was validated by crystallizing membrane proteins in lipidic mesophases, including LCP. This system allows for accurate dispensing of LCP material in nanoliter volumes, prevents inadvertent phase transit...
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The in meso method for growing crystals of membrane proteins uses a spontaneously forming lipidic cubic mesophase. The detergent-solubilized protein is dispersed with lipid, typically monoolein, and in so doing the cubic phase self-assembles. A precipitant is added to trigger crystal nucleation and growth. The commercial screen solution series are convenient for use in crystallization trials. T...
متن کاملPreparation and Delivery of Protein Microcrystals in Lipidic Cubic Phase for Serial Femtosecond Crystallography
Membrane proteins (MPs) are essential components of cellular membranes and primary drug targets. Rational drug design relies on precise structural information, typically obtained by crystallography; however MPs are difficult to crystallize. Recent progress in MP structural determination has benefited greatly from the development of lipidic cubic phase (LCP) crystallization methods, which typica...
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ژورنال
عنوان ژورنال: Current Opinion in Structural Biology
سال: 2011
ISSN: 0959-440X
DOI: 10.1016/j.sbi.2011.06.007